鲍鱼脏器粗多糖对糖尿病小鼠生理功能的影响

Impact of Crude Polysaccharides from Abalone Viscera on the Physiological Function of Diabetic Mice

摘要:

以一种鲍鱼内脏粗多糖（AVP）为实验原料，以其对小鼠降血糖及糖耐量改善效果、抗氧化效果、脏器保护效果为依据，研究其对四氧嘧啶诱导糖尿病小鼠生理功能的影响。结果表明：多糖组能显著控制小鼠消瘦，高、中剂量具极显著效果（P<0.01），给药3周后体重分别为33.96 g和32.95 g；AVP能明显降低糖尿病小鼠血糖值,中剂量（200 mg/kg）降糖效果最好，给药3周后空腹血糖降至15.45 mmol/L，且在一定范围内能改善小鼠糖耐量异常；治疗3周后，多糖组及药物组小鼠血清SOD和GSH-PX活性增加，MDA含量减少，中剂量组SOD、GSH-PX及MDA含量为155.25 U/mL、529.63 μmol/L、14.41 μmol/L，与模型组相比有极显著差异（P<0.01），揭示了AVP能清除体内过多自由基、提高糖尿病小鼠抗氧化酶活性，促进受四氧嘧啶损害细胞的修复和再生；与模型组相比，多糖组和药物组均可降低糖尿病小鼠脏器指数，中剂量组肝脏、肾脏和脾脏指数分别为5.23、0.94、0.27，具有显著差异（P<0.05）。

Abstract:

In this study, effects of abalone viscera polysaccharide (AVP) on the glucose tolerance, serum glucose level, the antioxidant ability and organ protection of diabetic mice were investigated, in order to explore the AVP impact on the physiological function of diabetic rats induced by alloxan. Results showed that, the AVP model groups controlled the weight of mice significantlywhere. Especially for the high and middle dose groups (p<0.01), the weight of mice were 33.96 g and 32.95 g respectively after three weeks treatments. AVP lowered the blood glucose in diabetic mice and improved impaired glucose tolerance in a certain extent. The middle dose group (200 mg/kg) had the best effect, decreasing blood glucose to 15.45 mmol/L after three weeks. Meanwhile, the activity of SOD and GSH-Px promoted obviously, while the MDA content in blood serum reduced after three-week dosage. The activity of SOD, GSH-Px and MDA content of middle dose group were 155.25 U/mL, 529.63 μmol/L and 14.41 μmol/L, respectively, with significant difference compared to the control group (p<0.01). Moreover, AVP protected liver, kidney and spleen of sick mice, the viscera index of middle dose group were 5.23, 0.94 and 0.27, respectively, showing significant difference (p<0.05).