利用少动鞘氨醇单胞菌酶解制备棉籽壳低聚木糖

Preparation of Xylooligosaccharides from Cotton Seed Huskby Sphingomonas paucimobilis 2 (1.) (2.) Abstract: Key words:

摘要:

本文研究了利用自筛菌株酶法制备棉籽壳低聚木糖的基本工艺。低聚木糖是主要的功能性食品添加剂，棉籽壳是生产低聚木糖的良好来源。因此，如何有效的从棉籽壳中提取低聚木糖成为亟待解决的问题。本研究中通过筛选鉴定（法国梅里埃生物自动识别系统）得到一株新的产内切型木聚糖酶的菌株-少动鞘氨醇单孢菌。通过酶解木聚糖工艺的优化，结果表明：当酶解温度为30 ℃，酶解8 h，木聚糖酶的浓度15%，底木聚糖浓度为40 g/L时，低聚木糖的得率可达到53.20%，经HPLC分析，酶解野种木二糖和木三糖占低聚木糖总量的48.56%，低聚木糖占总糖的82%以上，以上研究可为工业生产低聚木糖工艺的优化提供依据。

Abstract:

As a kind of agricultural waste, cotton seed husk is a potential source of functional xylooligosaccharides. In this study, a new strain NS5, which could produce endo-xylanase, was screened by France Biomerieux microorganism automatic identification system and preliminarily identified as Sphingomonas paucimobilis. The optimum enzymolysis conditions for xylooligosaccharides production were obtained as follows: temperaure 30 ℃, time 8 h, xylanase concentration 15 % and xylan concentration 40 g/L. Under these conditions, the yield of xylooligosaccharides was 53.20%. By analysis with HPLC, xylooligosaccharides was accounted for more than 82% of the total sugar, 48.56 % of which were identified as xylobiose and xylotriose. These results provided information for xylooligosaccharides preparation from cotton seed husk for future use in food industry.