Abstract:Clenbuterol Hydrochloride, categorized as brown meat essence, is prohibited in China. Clenbuterol is detected by two methods of continuous and rapid according to the law of immune response. The methods are based on surface plasmon resonance by using the biological chip. They are capable of detecting anti-Clenbuterol without labeling. The continuous method renews the chip by injecting the sample and the PBS buffer solution alternatively and injecting SDS-HCl after detection. The method can increase the detection times and extend the life cycle of the biological chip, which is suitable for detecting anti-Clenbuterol, screening anti-Clenbuterol, studying immunoreaction kinetics and establishing standard curves. In addition, the rapid method can adjust the scan angle dynamically in the process of biological chip preparation and immune response detection, which can effectively improve the sensitivity of the detection, remove the redundant data simultaneously, and achieve the detection limit as high as 2 mg/L. Clenbuterol sample (2.75 μg/L) extracted from pork was detected. The proposed methods showed high sensitivity, user-friendly control, rapid detection, label-free analysis, low cost, simple construction, and environmentally friendly equipment. Both methods could achieve the real-time detection of substantial samples.
