SPE-HPLC紫外双波长测定水果中10种农药残留
DOI:
CSTR:
作者:
作者单位:

作者简介:

作者简介:邵金良(1979-),男,助理研究员,主要从事农药残留分析检测技术研究 通讯作者:刘宏程(1975-),男,博士,研究员,主要从事农产品安全评价及风险评估

通讯作者:

中图分类号:

基金项目:

云南省科技创新强省计划项目(2009AB002);云南省社会发展科技计划(2012CA005)


Determination of 10 Pesticide residues in Fruits by HPLC with SPE and UV Double Wavelength
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    建立了固相萃取(SPE)-高效液相色谱(HPLC)紫外检测器双波长同时测定草莓、桃和苹果中吡虫啉、啶虫脒、多菌灵、甲基硫菌灵、嘧霉胺、除虫脲、灭幼脲、辛硫磷、苯醚甲环唑和阿维菌素10种农药残留的分析方法。水果样品用乙腈作为提取溶剂经高速匀浆提取,提取液经LC-NH2固相小柱净化富集,以甲醇、水和乙腈作为梯度洗脱的流动相,采用Shiseido CAPCELL PAK MG ⅡC18色谱柱(250 mm×4.6 mm,5 μm)分离,选择270 nm和245 nm双波长进行目标化合物的检测。结果表明:10种农药在0.05~1.0 mg/kg范围内具有良好的线性关系(0.9834~0.9992),检出限为0.005~0.030 mg/kg,定量限为0.020~0.098 mg/kg。0.05、0.10、0.20 mg/kg添加水平的回收率为69.82~88.95%,86.77~110.61%和89.74~110.62%;相对标准偏差(RSD)为6.52~13.24%,5.21~10.32%和3.56~9.88%。与其他方法相比,具有简便、准确、节约试剂和能耗低等优点,适合水果中10种农药残留的同时测定及定量分析。

    Abstract:

    HPLC with solid-phase extraction (SPE) and UV double wavelength was presented to analysis of imidacloprid, acetamiprid, carbendazim, thiophanate-methyl, primigravida, diflubenzuron, chlorbenzuron,phoxim, difenoconazole and abamectin pesticide residues in strawberry, peach and apple. Sample preparation involves extraction with acetonitrile, and then purification and enrichment by LC-NH2 solid. Chromatographic analysis was performed on Shiseido CAPCELL PAK MG ⅡC18(250 mm×4.6 mm, 5 μm)HPLC column using methyl alcohol, water and acetonitrile as gradient eluents. Dual wavelength at 270 nm and 245 nm was conducted to detect target compounds. All the ten pesticides ranging from 0.05 mg/kg to 1.0 mg/kg had a good linear relationship (0.9834~0.9992). And the limits of detection and quantification for the ten pesticides were 0.005~0.030 mg/kg and 0.020~0.098 mg/kg, respectively. The average recoveries obtained from samples spiked with standards at concentrations of 0.05, 0.10 and 0.20 mg/kg were in the range of 69.82~88.95%,86.77~110.61% and 89.74~110.62%, respectively, with relative standard deviations (RSD) of 6.52~13.24%, 5.21~10.32% and 3.56~9.88%, respectively. This method can be applied to determine and quantitative analysis of 10 pesticide residues in fruits, which was more convenient operation, higher sensitivity, higher accuracy and better repeatability than other methods.

    参考文献
    相似文献
    引证文献
引用本文

邵金良,汪禄祥,梅文泉,杜丽娟,陈兴连,樊建麟,刘宏程. SPE-HPLC紫外双波长测定水果中10种农药残留[J].现代食品科技,2013,29(10):2547-2553.

复制
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2013-06-18
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2013-10-28
  • 出版日期:
文章二维码
×
因办公室装修,期间暂时无法接听电话,如有事请QQ或邮件联系。信息咨询:QQ: 2553003667稿件处理1:QQ: 1542354573稿件处理2:QQ: 2195608851 财务咨询:QQ: 1347040116 Email:mfood@scut.edu.cn、mfood@foxmail.com