离子色谱-脉冲安培法检测卷烟主流烟气中的6种水溶性糖

Simultaneous Analysis of Six Water-soluble Sugars in the Main Stream Smoke of Cigarettes by Ion Chromatography with Pulsed Amperometric Detection

摘要:

阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖和果糖是卷烟主流烟气中的6种主要水溶性糖。采用高效阴离子交换色谱-脉冲安培检测法对这6种水溶性糖进行了分离测定。优化了样品提取方法。捕集在剑桥滤片上的主流烟气中的水溶性糖用20 mg/L NaN3水溶液超声提取45 min，在分析前无需进行其它前处理。用2.5 mmol/L NaOH作为流动相在CarboPac PA20色谱柱上实现了对6种糖的有效分离。6种水溶性糖的的检测限在2.47~9.82 ?g/L之间，线性相关系数均大于0.999，日内精密度小于5.0%，日间精密度小于6.9%，高、中、低三种添加水平的回收率在97.1%~102.4%之间。方法成功应用于多个牌号卷烟样品的检测。在所测定的样品中，6种水溶性糖的含量在36.17 ?g/支和119.5 ?g/支之间。果糖含量最高，为24.63~74.66 ?g/支。其次为葡萄糖，为8.09~38.09 ?g/支。果糖和葡萄糖占了6种糖总量的90.5~94.7%。

Abstract:

Arabinose, galactose, glucose, xylose, mannose and fructose are the six major water-soluble monosaccharides in the main stream smoke of cigarette. These sugars were separated and determined by high-performance anion exchange chromatography with pulsed integrated amperometric detection. Soluble sugars of the main stream smoke trapped in a Cambridge filter were extracted with 20 mg/L sodium azide by ultrasonic wave for 45 minutes. Six sugars were effectively separated by using 2.5 mmol/L NaOH as eluent on an anion exchange column -CarboPac PA20. The detection limits were between 2.47~9.82 ?g/L and the linear correlation coefficients were above 0.999 in all cases. The intra-day and inter-day precision were below 5.0% and 6.9%, respectively. The recoveries of three spiked levels ranged from 97.1% to 102.4%. The established method was successfully applied in the detection of cigarette samples of various brands. The total contents of six sugars within the tested samples were 36.17~119.5 ?g/cigarette. Fructose had the highest content with the values between 24.63~74.66 ?g/cigarette. And glucose showed the second highest content, which took up 8.09 to 38.09 ?g/cigarettes. Both fructose and glucose accounted for 90.5~94.7% of the total contents of six sugars.