热带水果多酚提取物的抗氧化和抗增殖活性研究

Antioxidant and Antiproliferative Activities of Polyphenol Extract from 12 Tropical Fruits

摘要:

为明确12种热带水果多酚提取物的总酚含量、总抗氧化能力和抗肿瘤细胞增殖活性，分别采用Folin-Ciocalteu法确定了水果提取物的总酚含量，采用ORAC和FRAP的方法确定了其抗氧化能力，采用MTT的方法确定了其抗人肝癌细胞HepG2增殖的活性。结果显示，12种水果提取物的总酚含量为26.17~229.67 mg GAE/100g 鲜重，最高杨桃的总酚含量为最低鳄梨的8.78倍；ORAC和FRAP抗氧化值分别为607.05~2631.17 μmol TE/100g 鲜重和462.12~1067.92 μmol TE/100 g鲜重，杨桃具有最高的ORAC和FRAP抗氧化值，分别是最低木瓜的4.33倍和鳄梨的2.31倍；11种水果提取物抑制肿瘤细胞HepG2增殖的IC50值为31.79~66.93 mg/mL，抑制活性最弱木瓜的IC50值为最强杨桃的2.11倍。水果提取物的总酚含量与其ORAC抗氧化值（R2＝0.7839）、FRAP抗氧化值（R2＝0.7636）和抑制HepG2细胞增殖的IC50值（R2＝0.8847）之间具有显著的相关性。

Abstract:

To evaluate the phenolic contents, antioxidant and antiproliferative activities of phenolic extracts from 12 tropical fruits, the total phenolic content was determined by Folin-Ciocalteu method and the antioxidant activity was measured with oxygen radical absorbance capacity activities (ORAC). The ferric reducing ability of plasma (FRAP), and the antiproliferative activity against human hepatoma cells (HepG2) were analyzed by MTT assay. It was founda that the total phenolic contents ranged from 26.17 to 229.67 mg gallic acid equivalents (GAE) per 100g fresh weight (FW). Averrhoa carambola had the highest phenolic content, being of 8.87-fold higher than Persea americana which had the lowest phenolic content. The ORAC and FRAP values were in the ranges of 607.05~2631.17 μmol TE/100g FW and 462.12~1067.92 μmol TE/100g FW, respectively. Averrhoa carambola showed the highest ORAC value which was 4.33 times higher than Carica papaya, and the higherst FRAP value which was 2.31 times higher than Persea Americana. The limits of IC50 values against HepG2 cells were from 31.79 to 66.93 mg/mL. Carica papaya had the lowest antiproliferative activity, with a 2.11-fold higher IC50 value compared to Averrhoa carambola with the highest antiproliferative activity. In addition, the total phenolic contents showed significant correlations with ORAC (R2＝0.7839), FRAP (R2＝0.7636) and IC50 values against HepG2 cells (R2＝0.8847).