橙皮苷/羟丙基-β-环糊精包合物的理化性质研究

Physicochemical Properties of the Complex of Hesperidin and Hydroxypropyl-β-cyclodextrin

摘要:

橙皮苷具有抗氧化、抑菌、降血压、抗病毒、抗肿瘤及提高机体免疫力等多种生物活性功能，在功能性食品，医药和化妆品领域具有良好的应用前景，但由于其在水中的溶解度过低，限制了它的广泛应用。本研究采用溶剂法制备了橙皮苷/羟丙基-β-环糊精包合物，以提高橙皮苷的水溶性，采用紫外（UV）、红外（IR）、扫描电子显微镜（SEM）、差示量热扫描（DSC）、X-射线衍射（XRD）等波谱分析技术对该包合物的理化性质进行了研究。结果表明，橙皮苷与羟丙基-β-环糊精包合后，其物相发生了重大改变，橙皮苷以无定形状态完全分散在羟丙基-β-环糊精中，二者以氢键或范德华力等非共价键形式相结合。通过与羟丙基-β-环糊精的包合，橙皮苷在30 ℃水中的溶解度也从34.68 μg/mL增加至2049.20 μg/mL，其水溶性和稳定性得到了显著提高。

Abstract:

Hesperidin possesses many bioactivities, such as antioxidant, antibacterial, lowering blood pressure, antiviral, antitumor and improving immunity, which can be used in functional food, medicine and cosmetics. But its poor solubility in water limited its wide applications. In this study, the complex of hesperidin and hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared to improve the water solubility of hesperidin by solvent method. Meanwhile, the physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and X-ray diffractometry (XRD). The results showed that the physical phase of hesperidin was significantly changed . It was completely dispersed in HP-β-CD matrix after inclusion. Hesperidin and HP-β-CD were combined with non-covalent bonds such as hydrogen bond and Van der Waals' force. HPLC analysis showed that the solubility of hesperidin in water at 30 ℃ was increased from 34.68 μg/mL to 2049.50 μg/mL