[关键词]
[摘要]
本文以黑大豆和黑芝麻为原料研制开发了黑大豆营养膳,并对其营养成分和缓解体力疲劳功能进行了评价。发芽黑大豆经挤压膨化后与黑芝麻混合调配、微波灭菌加工成营养膳,按国标规定检测其营养成分含量并进行营养评价。将营养膳添加入基础饲料,以15, 30 , 45 g/kg?bw 3种剂量连续喂养昆明种小鼠45 d,观察其对小鼠各项体力疲劳指标的影响。结果表明营养膳符合FAO/WHO推荐的优质蛋白模式,氨基酸评分、化学评分、必需氨基酸指数分别为0.94、0.60、77.33。在缓解体力疲劳试验中,营养膳能延长小鼠负重游泳时间,提高肝糖原含量,降低血清尿素氮含量和血乳酸曲线下面积。其最适剂量为30 g/kg?bw。由此可见,黑大豆营养膳是一种优质蛋白补充剂,并具有缓解体力疲劳功能。
[Key word]
[Abstract]
A kind of nutrient was made from black soybean and black sesame through extruding, blending and grinding. Its nutrient components were determined with the national standards. Kunming mice were used to evaluate the anti-fatigue activities of the black soybean nutrient (black soybean nutrient was added in the feed) with different dosages(15, 30, 45 g/kg?bw) for 45 days, by measuring the swimming time, serum urea, hepatic glycogen and blood lactic acid. The results showed that the nutrient was in accordance with FAO/WHO high quality protein pattern. Amino acid score(AAS),chemical score(CS) and essential amino acid index(EAAI) of the black soybean nutrient was 1.02,0.65 and 78.77, respectively. Black health nutrition paste increased the swimming time of the mice and promote the level of their hepatic glycogen. In addition, the nutrient decreased the serum urea content and the area under the curve of blood lactic acid. The optimal dosage of nutrition paste was 30g/kg?bw.This research showed that the black soybean nutrient was a good protein supplement with alleviating fatigue function.
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[基金项目]
“十二五”国家科技支撑计划项目(2012BAD33B10;2012BAD37B08)