黄曲霉毒素B1致癌毒性相关基因的生物信息学分析

Bioinformatic Analysis of Genes Relative to the Carcinogenic Toxicity of Aflatoxin B1

摘要:

黄曲霉毒素是广泛存在于食品中的对人类危害最大的、最常见的霉菌毒素，其中黄曲霉毒素B1是致癌毒性最强的一种。为了筛选与黄曲霉毒素B1致癌作用密切相关的基因，利用生物信息学技术对从GEO数据库中下载的三组相关的人基因组芯片实验数据集作系统的聚类分析，并对候选基因进行初步的数据库检索。首先选取数据集中表达比值相差2倍以上的基因做聚类分析，然后对聚集结果中表达趋势最为接近的差异基因作生物信息学数据库的检索，最后发现KRT15、PCNA、MMP1等多条基因与黄曲霉毒素B1关系紧密。通过对三组基因组芯片数据的生物信息学分析，找出了多条可能是黄曲霉毒素B1致癌毒性相关的关键基因，为进一步研究黄曲霉毒素诱导癌症发生、发展的具体机理提供了新的思路。

Abstract:

Aflatoxin is one of the most harmful mycotoxins existing extensively in daily food, among which carcinogenic toxicity of aflatoxin B1(AFB1) showed the highest carcinogenic toxicity. To screen genes strongly associated with the carcinogenic effects of AFB1, three AFB1-related human genome microarray datasets were downloaded from GEO database and analyzed by clustering and bioinformatic retrieving in this study.A set of genes were selected for clustering, which expression differences were greater than 2 fold in three microarray datasets. Then the genes expressed in accordant trend in the cluster analysis were screened and the genomic information about those genes and the association with AFB1 were determinated. Several genes such as KRT15, PCNA and MMP1 were identified as key AFB1-related genes through bioinformatics analysis on three relative microarray datasets,. These data may be benefit for further investigations of the genesis and progression of the AFB1-induced cancer．