新型常压室温等离子体（ARTP）快速诱变高产苏氨酸的突变株

A Mutant Strain with High Threonine Yield Obtained by Rapid Mutation Using Novel Atmospheric and Room Temperature Plasmas

摘要:

本文采用新型常压室温等离子体（ARTP）生物诱变育种技术，基于48孔板高通量筛选获得一株L-苏氨酸产量明显提高的突变株（1905#）。相比较与出发菌株，在相同的发酵培养条件下，1905#突变株鲁棒性较好、生长速率加快，最佳温度（37℃）和pH(7.2)基本一致，能耐受更低的pH值。孔板初筛结果表明苏氨酸浓度提高了133.59%，并且对摇瓶发酵过程进行了研究，相比较与出发菌株糖代谢速率加快，产酸强度提高，苏氨酸浓度提高了99.6%。经50次传代，1905#突变株表现出很好的遗传稳定性，为产业化水平的提高提供了坚实的理论基础和数据参考。

Abstract:

To obtain mutants with high threonine productivity, atmospheric and room temperature plasmas (ARTP) mutation technique was used and combined with high throughput screening based on 48 well plates. Mutant 1905# was screened with high threoine productivity. Compared with original strain under the same fermentation conditions, the mutant 1905# showed better robustness and faster growth rate. The mutant showed similar optimum temperature (37 ℃) and pH (7.2) to the original strain, but it can tolerate lower pH. In the preliminary plate screening, the threonine concentration increased by 133.59%. For the flask fermentation process, the mutant 1905# showed higher glucose consumption and enhanced threonine productivity The threonine concentration by the mutant was increased by 99.6% compared with that by the original. The 1905# mutant showed high genetic stability after 50 subcultures, offering theoretical reference for further increasing threonine industrialization level