滑子菇多糖的免疫活性及抗肿瘤作用

mmunological and Antitumor Activities of the Polysaccharides from Pholiota nameko

摘要:

本文选用豚鼠血清为补体的模型进行体外抗补体实验的检测、采用体外对小鼠腹腔巨噬细胞RAW264.7的毒性试验、腹腔巨噬细胞吞噬中性红活性能以及刺激巨噬细胞产生NO和H2O2 的能力来研究滑子菇多糖的免疫活性；通过对人体前列腺癌22Rv.1细胞和人体肝癌Hep 2B细胞增殖的抑制作用试验对滑子菇多糖的抗肿瘤活性进行研究。实验显示，滑子菇多糖具有较好的抗补体活性，并且活性随多糖浓度增大而增强，多糖浓度达到12.5 mg/mL活性变化不大，多糖浓度在0.005~0.5 mg/mL范围在一定程度上能够促进巨噬细胞的增殖，能增强腹腔巨噬细胞吞噬中性红活性能，并且促进巨噬细胞分泌NO和H2O2能力，表明滑子菇多糖具有较好的免疫活性；对人体前列腺癌细胞和人体肝癌细胞这两种癌细胞具有较强的抑制作用，并且活性与浓度呈现量效关系。

Abstract:

Guinea pig blood serum was chosen as the complement to study anticomplement research and mouse peritoneal macrophages RAW264.7 was used for toxicity test. The capabilities of macrophage englobing neutral red and secreting NO and H2O2 were investigated to study Pholiota nameko polysaccharides (PNP) immunocompetence. The antitumor activities of the PNP were studied through prostatic cancer 22Rv.1 cells and human hepatoma Hep 2B cells inhibition. The results showed that the PNP owned good anticomplement activity and activity of PNP was strengthened with its concentration increased.when increasing the polysaccharides to 12.5 mg/mL above. In concentration of 0.005~0.5 mg/mL, PNP could promote the proliferation of macrophages and peritoneal macrophage phagocytic ability, and increased NO and H2O2 secreting, indicating that PNP had good immunological activity. PNP had strong inhibition to the prostate cancer cells and liver cancer cells, with a concentration-response relationship.