富锗树舌胞外多糖的体外抗氧化活性研究

In Vitro Antioxidative Activity of Germanium-rich Exopolysaccharides from Ganoderm Applanatum

摘要:

利用树舌为材料通过液体深层富锗培养获得富锗胞外多糖，测定了树舌富锗胞外多糖中锗含量及其抗氧化活性，即清除氧自由基、羟自由基、DPPH自由基、亚硝酸盐的能力和总还原力，并以维生素C（Vc）作为阳性对照。实验结果表明：树舌富锗培养基内锗质量浓度为150 μg/mL时，可显著提高胞外多糖的产量，使胞外多糖产量达到1.12 g/L，比对照组提高36.62%。同时胞外多糖中的有机锗含量也得到显著提高达到0.26 mg/g，比对照组提高116.67%。有机锗对提高胞外多糖清除氧自由基、羟自由基、DPPH?、亚硝酸盐的能力及总还原力有显著的促进作用，在多糖浓度为2.5 mg/mL时，分别比对照提高25.52%、31.83%、26.95%、16.38%和10.36%。富锗树舌胞外多糖抗氧化活性与多糖浓度呈明显的剂量-效应关系。

Abstract:

Germanium-rich exopolysaccharides (GEPS) was prepared via submerged fermentation of Ganoderm applanatum, and the antoxidative activity of GEPS in vitro was evaluated by determining its capability in eliminating superoxide anion free radical, hydroxyl radical, DPPH?and sodium nitrite. Also, those various antioxidant activities were compared to Vc. Results showed that GEPS reached 1.12 g/L, being of 36.62% higher than those of the control. The organic Germanium reached 0.26 mg/g, being of 116.67 % higher than those of the control when the concentration of GeO2 in culture medium was 150 μg/mL. The organic Germanium could greatly enhance the free scavenging effective to superoxide anion free radical, hydroxyl radical, DPPH?, sodium nitrite and deoxidization capacity of exopolysaccharides, being of 25.52%, 31.83%, 26.95% and 16.38%, respectively, higher than those of the control when GEPS was 2.5 mg/mL. The antoxidative activity of GEPS displayed a dose-response relationship with its concentrations.