高效液相色谱法同时测定果蔬中多种防腐剂

Simultaneous Determination of Multiple Preservatives in Fruits and Vegetables by HPLC

摘要:

建立了高效液相色谱法同时测定水果及蔬菜中联苯、邻苯基苯酚、噻苯咪唑、抑霉唑、多菌灵、桂醛、乙萘酚、2, 4-二氯苯氧乙酸的方法。样品用碱性乙醚提取，浓缩后甲醇定容，经ODS-SP C18（4.6×150 mm，5 μm）柱分离，以甲醇-磷酸盐缓冲液（pH=6.8）为流动相，流速0.8 mL/min梯度洗脱，二极管阵列检测器（DAD）检测。此方法相关系数范围0.9991~0.9997，加标回收率范围88.0~97.4%，相对标准偏差0.53~3.9%。实验结果表明，该方法操作简单，结果灵敏准确，适用于水果及蔬菜中多种防腐剂的同时测定。

Abstract:

A HPLC method was developed for the determination of multiple preservatives in fruits and vegetables, including ortho-phenyl phenol, thiabendazole, imazalil, carbendazim, cinnamaldehyde, 2-naphthol, and 2,4-dichlorobenzene oxygen ethanoic acid. Samples were extracted by alkaline ether. The extract was dissolved in methanol and finally injected into the HPLC system after the extraction solution was evaporated to dry on a rotary evaporator. The separations were effected by the gradient elution mode using a mobile phase composed of methanol-phosphate buffer (pH 6.8) at the flow of 0.8 mL/min. The eight preservatives were separated on an ODS-SP C18 column and then detected using a DAD detector with detection wavelength of 190~400 nm. The correlation coefficient of this method ranged from 0.9991 to 0.9997. The recoveries at spiked levels ranged from 93.4% to 97.4% and the relative standard deviations (RSD) ranged from 0.53 to 1.96. The results demonstrated that this method was simple, sensitive and accurate, which had a promising potential for practical applications in the determination of preservatives.