覆盆子中有机酸含量的测定

Determination of the Contents of Organic Acids of Raspberry

摘要:

覆盆子为药食两用果实，具有抗衰老、调节生殖系统、促进细胞免疫机能、减肥之功效。本研究利用HPLC法对覆盆子干果中有机酸含量进行测定，测定的色谱条件为：柱温30 ℃，流动相为0.05 mmol H2SO4，流速0.5 mL/min，检测波长210 nm，进样量10 μL，色谱柱为C18柱，在设定的色谱条件下覆盆子干果处理液峰面积与有机酸标品线性关系良好，相关系数在0.9998~0.9999之间，精密度实验各有机酸RSD值在0.30~0.90%之间，重现性实验各有机酸RSD值在0.42~3.69%之间，加标回收率在97.86~102.35%之间，此方法用于测定覆盆子干果中有机酸含量准确、可行。

Abstract:

As an edible Chinese herb, raspberry could improve overall energy, vitality aphrodisiac and immune booster. A method for determining organic acids in Raspberry by HPLC was developed and the best HPLC determination conditions with a C18 column were determined as follows: column temperature 30 ℃, mobile phase 0.05 mmol H2SO4, flow rate 0.5 mL/min, detection wavelength 210 nm and injection volume 10 μL. Under the detection conditions. five organic acids could be successfully separated with good linear relationships (correlation coefficients above 0.9998). The precision RSD, repeatability RSD and recovery rates were of 0.30~0.90%, 0.42~3.69% and 97.86~102.35%, respectively. The method was convenient, rapid, accurate and applicable to determine organic acids in raspberry.