虎杖中白藜芦醇提取工艺研究

Extraction of Resveratrol from Polygonum cuspidatum

摘要:

白藜芦醇是一种重要的活性天然产物，本研究以虎杖为原料，研究了白藜芦醇的溶剂法和酶解法提取工艺，考察了料液比、提取时间、提取温度、pH、酶加入量等对提取得率的影响．通过正交实验优化了溶剂法和酶解法的最佳工艺参数．实验结果表明：溶剂法优化工艺为：pH 5，料液比1:65，提取温度是60 ℃，提取时间2 h；酶解法优化工艺为：pH 6，料液比1:40，酶解温度是60 ℃，酶解时间2 h，二者得率分别是：0.496%和0.942%。酶解法的得率大约是溶剂法的2倍，可以作为虎杖中白藜芦醇提取的优选方法。

Abstract:

Resveratrol is an important ntural compound with bioactivity. In this experiment, Polygonum cuspidatum was used as raw materials for extraction process f resveratrol. Effects of solid-liquid ratio, raw material particle size, ethanol concentration, extraction time, extraction temperature were investigated on the extraction rate. The orthogonal test was used to determine the optimum conditions with solvent and enzymatic extraction of resveratrol in Polygonum cuspidatum. The results showed that the best solvent extraction conditions were pH 5, solid-liquid ratio 1:65 (g:mL), temperature 60 ℃ and extraction time 120 min; The optimum conditions for enzymatic extraction were pH 6.0, solid-liquid ratio 1:40 (g:mL), hydrolysis temperature 60 ℃, and hydrolysis time 120 min. The results showed that, the yields of solvent and enzymatic extractionwere 0.496% and 0.942%, respectively. Compared with solvent method, extraction yield of resveratrol by using enzymatic method was increased by about 2 times. Enzymatic method can be used as the preferred method of extraction of resveratrol.