抗坏血酸及壳聚糖对大豆富硒作用的影响

Influence of Ascorbic Acid and Chitosan on Selenium Enrichment of Soybean

摘要:

本文通过比较经不同浸泡处理后，富硒大豆的萌发率、芽长、总硒含量等；研究了浸泡温度，浸泡时间，及浸泡液中亚硒酸钠浓度、抗坏血酸、壳聚糖等对大豆富硒作用的影响。通过碱提酸沉法提取得到富硒大豆蛋白，采用氢化物原子荧光光谱法测定其硒含量。结果表明，大豆富硒的最佳工艺条件为：浸泡温度为30 ℃，浸泡时间为8 h，浸泡液中亚硒酸钠浓度为60 μg/mL左右。在浸泡液中添加0.08%（m/V）的抗坏血酸对大豆的萌发和生长有利，但会影响大豆对无机硒的吸收和转化；若在浸泡液中同时添加0.1%（m/V）的壳聚糖，则可以克服上述不利影响，提高大豆对无机硒的吸收和转化。

Abstract:

In order to improve the selenium enrichment effect of soybean, the influence of soaking condition, ascorbic acid and chitosan on the sprouting rate and sprout length of the selenium enrichment soybean were investigated in this study. Results indicated that the ascorbic acid will improve the germination and growth of soybean seeds, and will disadvantage to the selenium enrichment of soybean, but after adding chitosan to the soaking solution, the negative influence of the ascorbic acid will extinction. The best formula of soaking solution contained 60 μg/mL Na2SeO3, 0.08% ascorbic acid and 0.1% chitosan. The best soaking time and temperature were 8 h and 30 ℃, respectively. After processing, total selenium content and the isolated protein of the selenium-enriched soybean reached 27.31 μg/g and 31.95 μg/g, respectively.