一株地衣芽孢杆菌噬菌体的分离及其性质分析

Isolation and Characterization of Bacillus licheniformis Phage

摘要:

从生产碱性蛋白酶的车间附近的下水道淤泥和后处理车间空气中分离得到了一株噬菌体。电镜观察表明该噬菌体有多面体的头部和细长的尾部组成，有着长尾病毒科噬菌体的形态特征。在供试菌种中，该噬菌体以地衣芽孢杆菌为宿主，对其生理特性研究发现，该噬菌体在pH 5~10之间有着很好的稳定性，在高温下有一定的稳定性，且金属离子可以促进其对宿主菌的侵染。根据实验结果绘制出噬菌体的一步生长曲线，可知感染宿主菌的潜伏期约为40 min，爆发期约为110 min。对噬菌体核酸性质的分析发现，其遗传物质为双链线状DNA，其分子量大约为36 kb。

Abstract:

A virulent bacteriophage of Bacillus licheniformis was isolated from the nearby drainage and air environment of alkali protease producing factory. Electronic microscopy showed that the phage had an isometric head (59 nm in diameter) and a long non-contractile tail (132 nm in length), which indicated the characters of Siphoviridae family. Bacillus licheniformis was the host cell for the phage in the test. The study revealed that the phage had good stability at pH 5~0 and certain stability under high temperature. The invasion to the host cell could be accelerated by metal ion. One-step growth kinetics showed that the latent period of the invasion was about 40 min, and the rise period was about 110 min. Double-stranded linear DNA was the genetic material of the phage, with a molecular weight of about 36 kb.