乳杆菌抑菌和粘附性能的研究

Antimicrobial and Adhesion Properties of Four Lactobacillus Strains

摘要:

本文以4株乳杆菌为研究对象，标准益生菌L. rhamnosus GG作为对照。研究它们对低酸和高浓度胆盐的耐受性，对抗生素的敏感性，对食源性病原菌的抑制能力以及对Caco-2细胞的粘附性。结果表明，其中Lactobacillus casei 142，L. plantarum 89的耐酸耐胆盐性能较好。四株乳杆菌对Listeria monocytogenes，Escherichia coli，shigella sonnei都有较好的抑制作用，同时它们对本研究中的5种抗生素都具有敏感性。本文采用Caco-2细胞模型，研究乳杆菌对Caco-2细胞的粘附能力，结果发现L.plantarum 89， L.plantarum 27和L.plantarum 13在Caco-2细胞上的粘附能力强（>20 cfu/cell），L. rhamnosus GG的粘附数为8（cfu/cell），而L. casei 142则不具有粘附性能。

Abstract:

The present work explored the antimicarobial and adhesion property of four lactobacillus strains with probiotic potential and compared with the typical probiotic L. rhamnosus GG, by investigation of their acid and bile torelance, antibiotic susceptiblity, antagonism against important gastrointestinal pathogens and their ability of adhere to Caco-2 cell. Lactobacillus casei 142 and L. plantarum 89 can survived in both the acidic and bile salt environments. All lactobacillu strains tested were sensitive to the antibiotics used in this investigation and had strong antimicrobial effect against the pathogens. L.plantarum 89, L.plantarum 27 and L.plantarum 13 showed strong adhension abilities, with their adhension capacity to the Caco-2 cell being of ＞20 cfu/cell. However, L. casei 142 showed no adhesion ablity to Caco-2 cells. The results suggested the probiotic potential of the four strains.