臭灵丹中黄酮类化合物的鉴定及抗氧化活性的研究

Structural Identification and Antioxidant Activity of the Flavones from Laggera pterodonta

摘要:

采用乙醇室温浸泡臭灵丹粉，60 ℃减压浓缩后，用石油醚除脂，然后用乙酸乙酯萃取，得到臭灵丹的乙酸乙酯萃取物；利用硅胶柱层析法对乙酸乙酯萃取物进行分离，得到两个单体化合物；利用核磁共振技术鉴定单体化合物为黄酮类化合物；采用高效液相色谱法检测单体化合物的纯度均大于95%；以维生素C为对照，采用清除DPPH自由基试验方法研究单体化合物的抗氧化活性。结果显示臭灵丹中含有的黄酮类化合物为洋艾素和金腰乙素，它们均有明显的抗氧化作用，其体系终浓度IC50分别为9.88 ?g/mL和10.28 ?g/mL，但其抗氧化活性均弱于维生素C。

Abstract:

The plant of Laggera pterodonta was powered, extracted with ethanol and purified by silica gel column chromatography. The purity of the compounds from the plant was investigated by HPLC and their structures were identified on the basis of spectral analysis. Then the antioxidant activity of the compounds was evaluated by DPPH radical scavenging assay. Artemitin and chrysosptertin B were separated from Laggera pterodonta, which showed antioxidant activity lower than vitamin C.