高效液相色谱法同时测定人参中多菌灵和甲基硫菌灵农药残留的含量

Simultaneous Determination of Residual Carbendazimin and Thiophanate-methyl in Ginseng by High Performance Liquid Chromatography

摘要:

建立了高效液相法同时测定中药材人参中多菌灵、甲基硫菌灵农药残留含量农药残留量方法。色谱条件：Diamonsil C18色谱柱，流动相为甲醇:水，梯度洗脱,检测波长为285 nm。结果平均回收率多菌灵为85.73%，RSD为2.07% (n=6)；平均回收率甲基硫菌灵为85.83%，RSD为2.39%(n=6)。多菌灵在0.2~1.0 μg时，r=0.9990，呈良好的线性关系，甲基硫菌灵在0.2~1.0 μg时，r=0.9993，呈良好的线性关系。本方法简便、快速、适合同类中药材中多菌灵、甲基硫菌灵农药残留量测定与安全监控。

Abstract:

A High performance liquid chromatography (HPLC) method was developed in this paper for simultaneous determination of residual carbendazimin and Thiophanate-methyl in Ginseng, a Chinese traditional medicine.The HPLC analysis of the samples were carried out using methanol-water as mobile phase on a Diamonsi C18 column at 285 nm. The average recovery and RSD for Carbendazimin were 85.73% and 2.07% (n=6), respectively. The average recovery and RSD for Thiophanate-methyl were 85.83% and 2.39% (n=6), respectively. The standard curve of carbendazim was linear in the range of 0.2~1.0 μg (R=0.9990)and the standard curve of Thiophanate-methyl was linear in the range of 0.2~1.0 μg (R=0.9993). This method was simple, rapid and reliable1, which is suitable for the determination and safe control of residual carbendazimin and Thiophanate-methyl in Chinese traditional medicines.