同位素比率质谱技术对蜂蜜掺假的鉴别

Identification of Honey Adulteration by Isotope Ratio Mass Spectrometry

摘要:

本文扩展了同位素比率质谱联用技术的应用，完善了液相色谱联用同位素比率质谱（LC-IRMS）测定蜂蜜中多种糖组分δ13C值的方法。通过对3种糖浆、3种掺糖蜜和纯正蜂蜜测定结果和图谱的多重比较，发现单纯的使用元素分析联用同位素比率质谱(HT-IRMS)进行测定，无法完全鉴别出掺糖蜜，但是结合LC-IRMS的测定结果则能有效的对以上3种方式的掺假进行鉴别。对6种特制蜂蜜进行测定并判定，判定结论与实际制样情况相符。该技术手段丰富了鉴别掺假蜂蜜的方法，具有较强的应用价值。

Abstract:

In this research, Liquid Chromatography - Isotope Ratio Mass Spectrometry (LC-IRMS) was applied toDetermination of honey adulteration by testing the value of δ13C of a variety of sugar components in honey. After the multiple comparisons of the measurements and the collection of plates of 3 kinds of syrup, doped molasses and pure honey, it was found that doped molasses could not be used to identify honey adulteration by only using Elemental Analysis-Isotope Ratio Mass Spectrometry (HT-IRMS).However, the 3 kinds of doped molasses could be measured effectively by the method of HT-IRMS combined with LC-IRMS. The technology enriched the identification method for adulterated honey.