褶皱假丝酵母脂肪酶催化反应条件的研究

Catalytic Activity of Candida rugosa Lipase

摘要:

本文研究了褶皱假丝酵母脂肪酶的催化反应条件。通过单因素试验研究了温度、缓冲液pH值、酶的浓度对酶活性的影响，并通过正交设计实验对单因素得到的结果进行优化，得到了褶皱假丝酵母脂肪酶的最适反应条件，即温度55 ℃、缓冲液pH值6.0、酶的浓度100 mg/mL，在此条件下，获得的100 mg/mL CRL酶的活力为39.78 mU，CRL酶适合在60 ℃以下温度环境中进行反应，并且适合保藏在pH值5.0~7.0的磷酸氢二钠-柠檬酸缓冲液中。

Abstract:

In this paper, the activity of Candida Rugosa lipase (CRL) in catalyzing hydrolysis reaction was investigated through single factor experiment. And the optimal reaction conditions for CRL catalyzed hydrolysis were obtained as follows: temperature 55 ℃, buffer pH 6.0, and enzyme concentration 100 mg/mL. Under these conditions, the activity of 100 mg/mL CRL was 39.78 mU. CRL was suitable for catalysis at temperatures below 60 ℃and was stable when stored in buffer solution at pH values of 5.0~7.0.