不同前处理方法对蔬菜中有机磷农药残留检测的影响

Effect of Different Pre-treatment Methods on Detection Results of Organophosphorus Pesticide Residue Vegetables

摘要:

改进蔬菜中有机磷农药残留检测的前处理方法，本实验采用国标方法、振荡提取法和超声波提取法，分别提取蔬菜中的农药残留；采用酶抑制法测定不同超声时间对蔬菜提取液中农残含量的影响；同时比较直接测定、活性炭吸附后过滤、0.45 μm滤膜过滤三种不同处理方法对结果的影响。结果显示，对蔬菜的有机磷农药残留提取液进行10 min超声提取后用0.45 μm滤膜过滤效果最佳，回收率最高（敌敌畏88.89%，乐果93.03%）。将此方法与国标法进行比较发现，此方法具有准确度高、回收率高、重现性较好等优点。

Abstract:

To improve pre-treatment methods in vegetables organophosphorus pesticide residue detection, GB method, oscillation extraction method and ultrasonic extraction have been used to extract the pesticide residue in vegetables; And then enzyme inhibition method was used to determine the effect of ultrasonic time and the affects of three different ways (direct measurement, active carbon adsorption after filtration and 0.45 μm film filtration) were compared. The best pre-treatment methods was ultrasonic extracting for 10 min followed by a 0.45 μm film filtration, with which the response rate was the highest. Compared with the GB method, the accuracy, recovery and reproducibility of this method were higher.