高效离子色谱法测定烟草及其制品中麦芽酚的含量

Determination of Maltol in Tobacco and Tobacco Products by High-performance Ion Chromatographic

摘要:

建立了使用阴离子交换分离柱，NaOH淋洗液，电化学检测器测定烟草制品中痕量麦芽酚的离子色谱检测方法。方法基于麦芽酚在NaOH溶液中转化为阴离子，使用CarboPac PA1型分离柱和NaOH淋洗液进行分离；利用酚羟基在玻碳电极上的特异响应，使用ED电化学检测器进行分析。在2.60?10–2~26.00 mg∕L浓度范围内，工作曲线的线性相关系数为0.9999，检出限为2.26 μg∕L；测定的整个过程可在15 min内完成。方法用于烟草样品的分析，结果令人满意，加标回收率在97%~102%之间。

Abstract:

A new method was developed for the determination of trace maltol in tobacco products using anion-exchange column, sodium hydroxide eluent and electrochemical detector. The sodium hydroxide was used to promote ionization of maltol to its anionic form. The maltol can be eluted within 15 min by anion-exchange column CarboPac PA1 with elution of NaOH. Detection occurred by measuring the current generated when the maltol anions were oxidized on a glass carbon electrode of ED electrochemical detector. The presented method showed good linear relationship (r=0.9999) for maltol in the range of 2.60×10–2~26.00 mg/L, the detection limit was 2.26 μg∕L. This method has been applied to the determination of maltol in tobacco samples successfully, and the recoveries vary from 97% to 102%.