超声波辅助提取荷叶和槐米黄酮的工艺研究

Ultrasonic Assisted Extraction of Flavonoids from Lotus Leaf and Sophora japonica

摘要:

黄酮类物质是一类具有极高经济价值的化合物，在各类农产品加工下脚料中含量丰富。研究以荷叶和槐米为对象，通过乙醇热浸和超声辅助提取黄酮类组分，采用正交实验优化提取工艺条件，并以高效液相色谱法进行提取物的成分及纯度测定。结果表明，超声辅助提取法优于乙醇热浸法。荷叶提取黄酮最佳工艺条件为：乙醇浓度70%，温度35 ℃，超声提取时间40 min，料液比1:40（m:v）；槐米提取黄酮的最佳工艺条件为：乙醇浓度70%，温度25 ℃，超声提取时间35 min，料液比1:40（m:V）。在最佳工艺条件下，提取物的主要成分为芦丁，荷叶芦丁得率为11.64%，槐米为15.47%。研究结果为提高农产品加工下脚料的附加值提供了依据。

Abstract:

Flavonoids is a class of compounds with high economic value, which is abundant in various types of agricultural processing waste. Flavonoids from Lotus leaf and Sophora japonica were extracted by ethanol hot-dip and ultrasonic assisted method. The extraction conditions were optimized according to the orthogonal experiment. Then the composition and purity of the extractions were determined by high performance liquid chromatography. Results showed that ultrasonic assisted extraction method was superior to ethanol hot-dip method. The optimum extracted conditions from Lotus leaf were as follows: ethanol concentration70%, temperature 35 ℃, extraction time 40 min, the ratio of material to solvent 1:40 (m:v). For the flavoids from Sophora japonica, the best extraction conditions were: the ethanol concentration70% , temperature 25 ℃, ultrasonic extraction time 35 min, and the ratio of material to solvent 1:40 (m:V). At the optimum conditions, the main component of the extractions was rutin and extraction yield reached 11.64% from the Lotus leaf and 15.47% from Sophora japonica. The research provided a basis to improve the value-added of agricultural processing waste.