辛硫磷的柱层析纯化及其结构表征

Purification and Structural Charateristics of Phoxim

摘要:

采用硅胶柱层析法对辛硫磷原药进行分离纯化，用红外光谱、核磁共振进行表征，高效液相色谱(HPLC)进行定量测定。结果表明，柱层析的最优洗脱剂为乙酸乙酯:石油醚=1:12。红外光谱、核磁共振都可以证明辛硫磷的提纯产品与其分子结构特征一致。高效液相色谱表明提纯的辛硫磷纯度可以达到98.60%。

Abstract:

Silica gel column chromatography was used to purify the phoxim technical. It can be characterized by IR spectra and 1H NMR. The content was determined quantitatively by HPLC. The results showed that the best mobile phase was Ethyl acetate-petroleum ether (1:12). IR spectra and 1H sNMR analysis showed that the phoxim was the same as its molecular structure. The purity of the achieved compound by HPLC was 98.6%.