超高效液相色谱-串联质谱法快速检测水产品中喹乙醇及其代谢物3-甲基-喹噁啉-2-羟酸的残留

Rapid Determination of the Residues of Olaquindox and 3-methylquinoline-2-carboxylic Acid in Aquatic Products by UPLC-MS/MS

摘要:

建立了水产品中喹乙醇（OQX）及其代谢物3-甲基-喹噁啉-2-羟酸（MQCA）的超高效液相色谱串联质谱快速测定方法。试样经乙酸乙酯+乙腈（1+1，V/V）液液萃取提取和净化后，BEH C18 UPLC进行分离，多反应选择离子检测。方法的回收率和变异系数分别在62.5%~91.4%和2.6%~11.8%之间。本方法对水产品中喹乙醇和3-甲基喹噁啉-2-羧酸的检测限均为1.0 μg/kg。

Abstract:

This study presented a ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for fast determination of the residues of olaquindox and 3-methylquinoline-2-carboxylic acid in aquatic products. Resident of olaquindox and 3-methylquinoline-2-carboxylic acid ware extracted by the liquid- liquid method using ethyl acetate+ acetonitrile（1+1, V/V）.After BEH C18 LC gradient elution separation,the analytes ware determined under multi-reaction monitoring (MRM) scan type with tandemmass analyzer using positive polarity mode. The recoveries are in the range of 62.5%~91.4%, the relative standard deviation are in the range of 2.6%~11.8%. The limit of detection (LOD) for olaquindox and methyl-3-quinoxaline-2-carboxylic acid in aquatic products was 1.0 μg/kg.