皱褶假丝酵母脂肪酶催化合成VE阿魏酸酯的研究

Candida rugosa Lipase Catalytalzed Synthesis of Alpa-tocopheryl Ferulate

摘要:

本文研究了反胶束体系中利用褶皱假丝酵母脂肪酶催化维生素E和阿魏酸乙酯合成维生素Ｅ阿魏酸酯的反应。通过单因素试验利用褶皱假丝酵母脂肪酶在AOT-异辛烷-水反胶束体系中催化阿魏酸乙酯和维生素E反应136 h生成VE阿魏酸酯，并研究了含水率、转速、温度、缓冲液pH值、AOT的浓度等反应条件对VE阿魏酸酯合成的影响。通过均匀设计法设计实验，并对结果进行二次多项式逐步回归分析，得到一组最优合成的反应条件，即含水率10、AOT浓度80 mmol/L、缓冲液pH值5.0、温度25 ℃、转速270 rpm，VE阿魏酸酯的得率为15.36%。

Abstract:

In this paper, Candida rugosa lipase (CRL) catalyzed reaction of vitamin E and ferulic acid ethyl ester were studied to synthesize alpa-tocopheryl ferulate in the reverse micelle system. Through single factor test and the use of the enzyme in AOT-iso-octane-water reverse micelle system, vitamin E and ferulic acid ethyl ester were catalyzed to synthesize alpa-tocopheryl ferulate after the 136 h reaction. And the influential factors of the synthetic product, such as the moisture content, speed, temperature, buffer pH, AOT concentration reaction conditions, were studied. Through the uniform design experiment and quadratic polynomial stepwise regression analysis of the results, the optimal reaction conditions of synthetic product were gained as follows: moisture content 10%, AOT concentration of 80mmol/L, the buffer pH5.0, temperature 25 ℃, speed 270 rpm, under which the VE Ferulate obtained was 15.36%.