南五味子有效成分提取及其在不同相系中的分布研究

Extraction of Effective Components from Schisandra sphenanthera Rehd.Et Wils and their Distribution State in Different Phase System

摘要:

以南五味子总木脂素、多糖为指标，研究南五味子有效成分的最优提取工艺条件及其在乙醇相和水相中的分布状态，采用紫外分光光度法和苯酚-硫酸法进行检测。结果表明：总木脂素最优提取工艺为乙醇浓度70%、料液比为1:9、提取3次、每次2 h，得率可达9.76 mg/g生药量；总多糖最优提取工艺为70%乙醇、料液比为1:7、提取3次、每次1.5 h，得率可达11.32 mg/g生药量。结果还表明，提取物经分离后在乙醇相中总木脂素和多糖含量均明显大于水相中含量。乙醇相中，总木脂素与多糖含量相当；水相中，多糖含量高于总木脂素量。

Abstract:

Using total Lignans and Polysaccharide of Schisandra sphenanthera Rehd. Et Wils as the indicator, study on the optimal extraction technology conditions of its effective components and distribution in ethanol and water phase. Using UV spectrophotometry and Phenol-Sulfuric acid method for testing, the results showed that the optimal extraction conditions of total lignans were alcohol concentration 70%, liquid ratio 1:9, extraction times 3, and extraction time 2 h, under which the extraction rate reached 9.76 mg/g. The optimal extraction conditions of polysaccharide were alcohol concentration 70%, liquid ratio 1:7, extractio times 3 and extraction time 1.5 h, under which the extraction rate reached 11.32 mg/g. The results also show that, after separation, the extract in ethanol phase was significantly greater than the total Lignans and polysaccharide content in aqueous media content. Total Lignans and polysaccharide content was similar in the ethanol phase. Polysaccharide content was higher than the total lignans content in the water phase.