超声辅助AOT反胶束提取花生蛋白后萃取工艺的优化

Backward Extraction of Peanut Protein through SDS Reverse Micelles Assisted by Ultrasound

摘要:

研究超声辅助AOT（二-(2-乙基己基)琥珀酸酯磺酸钠）/异辛烷反胶束体系萃取花生蛋白的后萃取过程，并分析各因素对蛋白后萃取率的影响，通过正交试验得到了最佳后萃取工艺条件为：缓冲溶液pH值为8.5、萃取时间为50 min、萃取温度为40 ℃、超声功率240 W、KCl浓度为1.5 mol/L，在此条件下，蛋白后萃取率为82.17±1.52%。

Abstract:

The backward of peanut protein was studied by AOT (sodium bis-(2-ethylhexyl) sulfosuccinate)/isooctane reverse micelles system from full-fat peanut powder with sonication. The main factors effecting reverse micelles extraction were analyzed. The orthogonal test design results showed that the optimum conditions of backward extraction were pH 8.5, extraction time 40 min, temperature 40 ℃, ultrasonic power 240 W, and KCl concentration 1.5 mol/L, under which the extraction rate was 82.17±1.52%.