苹果皮不同溶剂提取物抗氧化活性研究

Antioxidant Activities of Different Solvents Extracts from Apple Peels

摘要:

研究苹果皮不同溶剂提取物的抗氧化活性。依次用蒸馏水、60%乙醇、无水乙醇、乙酸乙酯提取苹果皮中的活性物质，用Folin-Ciocalteu法测定提取物中总酚含量，并以Vc为阳性对照，通过测定对羟自由基(?OH)、超氧阴离子自由基（O2-?）、亚硝酸盐（NO2-）的清除作用及总还原力，比较评价4种提取物的抗氧化活性。苹果皮60%乙醇提取物总酚含量显著高于其他提取物；在不同的抗氧化体系中，苹果皮的4种不同溶剂提取物均具有不同程度的抗氧化活性且与质量浓度呈明显的量效关系，60%乙醇提取物的抗氧化活性始终最强，其清除?OH和O2-?活性弱于Vc，而清除NO2-活性和还原能力较强且高于Vc。苹果皮抗氧化活性物质主要是60%乙醇提取物。

Abstract:

To explore the antioxidant activities of different solvent extracts from apple peels. The extracts of the antioxidants from apple peels were extracted with distilled water, 60% ethanol, anhydrous ethanol and ethyl acetate, respectively. Using Vc as a positive control, antioxidant activities of the extracts were tested and compared by hydroxyl radicals(?OH) scavenging activity, superoxide radical (O2-?) scavenging activity, nitrite radical (NO2-) scavenging activity and reducing power, and the total phenols contents of the extracts were also determined by the Folin-Ciocalteu method. The contents of total phenols from the 60% ethanol extract were significantly higher than those in the other three extracts. All the four extracts exhibited antioxidant capacities in a significant dosage-dependent manner. The 60% ethanol extract had the strongest antioxidant activities, the scavenging capacity to ?OH and O2-? of 60% ethanol extracts were weaker than Vc, but the scavenging capacity to O2- and the reducing power were stronger than Vc. The antioxidant substances from apple peels were main in the 60% ethanol extract.