采用超声波辅助提取法，对提取松针多糖的工艺条件进行研究。以水为提取溶剂，通过单因素试验研究5个因素：提取温度、超声功率、提取时间、料液比以及提取次数对松针多糖得率的影响。在单因素试验的基础上，通过正交试验对超声波辅助提取松针多糖的工艺条件进行优化。结果表明，最佳条件为：提取温度79 ℃，超声波功率400 W，提取时间30 min，料液比1:30 (g/mL)。在此最佳工艺条件下提取1次，松针多糖得率为4.15%。

Using the ultrasonic-assisted extraction method, process of extracting polysaccharides from pine needles was studied. With water as extraction solvent, 5 factors including extraction temperature, ultrasonic power, ultrasonic extraction time, ratio of solid to liquid and extraction times were studied for their effect on the yield of pine needles polysaccharides. On the base of the single factor experiments, the optimal processing conditions for the extraction of polysaccharides were obtained by orthogonal experiments. The results showed that the optimum parameters were: ultrasonic extraction time 30 min, extraction temperature 79 ℃, ratio of solid to liquid 1:30 (g/mL), and ultrasonic power 400 W. When extracting 1 times under these conditions, the yield of pine needles polysaccharides was 4.15%.

国家本科教学质量工程资助项目（TS11664）；韩山师范学院青年项目（LQ200906）