[关键词]
[摘要]
为探究黄褐毛忍冬总绿原酸含量、总黄酮含量与抗氧化活性相关性;利用50%甲醇恒温震荡提取黄褐毛忍冬花蕾中总绿原酸、总黄酮,紫外分光光度法测定其含量,DPPH比色法测定其抗氧化活性,采用SPSS软件对化合物含量与抗氧化活性进行相关性分析;结果表明:在0.01水平上总绿原酸含量、总黄酮含量与黄褐毛忍冬抗氧化活性呈正相关,相关数为分别为0.734和0.683,即绿原酸对黄褐毛忍冬的抗氧化活性贡献最大,其次是黄酮。
[Key word]
[Abstract]
For explore the correlation among the content of total chlorogenic acid, total flavonoids and their antioxidant activity of Lonicera fulvotmentosa, the total chlorogenic acid and flavonoids was obtained with rotary shaker extraction technique, their content and antioxidant activity detected by UV and DPPH methods, respectively. The correlation both the content of phenolic acids and their antioxidant activity have been analyzed using SPSS methods. The positive correlations have been shown significant both the content of total chlorogenic acid and flavonoids and their antioxidant activity of L. fulvotmentosa, the correlated factors were 0.734 and 0.683 at the 0.01 level. This means that the chlorogenic acid play important role for the antioxidant activity of L. fulvotmentosa, followed by was flavonoids.
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[基金项目]
国家自然科学基金(31060056);贵州师范大学博士科研项目(11904-05032110014);贵州省中药现代化专项(黔科合中药字[2011]5046号);贵州省科技基金项目(黔科合J字[2011]2368号);贵州省科技创新人才团队建设项目(黔科合人才团队[2009]4007号)