采用RP-HPLC法测定不同种荞麦、不同商品苦荞茶中芦丁的含量。以回流法提取，采用DIKMA diamonsil (4.6 mm ×250 mm, 5 μm)色谱柱，流动相为乙腈-0.1%磷酸水，柱温20 ℃，流速为1 mL/min，检测波长为255 nm，在该色谱条件下，芦丁分离效果好，线性范围为0.003976~0.5964 mg/mL（r=0.9999）。平均回收率为96.93%（n=6），RSD为2.72%。结果表明：苦荞中芦丁含量远远高于甜荞；不同生产地的苦荞茶中芦丁含量差异较大，可能与原料产地及制作工艺不同有关；不同类型苦荞茶商品中以全胚茶中芦丁含量最高。

A high-performance liquid chromatography method was established for the determination of rutin of buckwheat and its commodities. The extraction method was heat reflux, and rutin was separated on DIKMA diamonsil (4.6 mm ×250 mm, 5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid solution at the flow rate of 1.0 mL/min. The column temperature was 20 ℃ and the detection wavelength was set at 255 nm. The results indicated that rutin was basically isolated. The linear ranges was of 0.003976-0.5964 mg/mL (r=0.9999). The average recoveries was 96.93 % (n=6) with a RSD of 2.72 %. The results showed that the concentration of rutin of F. tataricum (L. ) Gaertn. was more higher than that of F. esculentum Moench,. The concentration of rutin of different producing area and preparation technique of buckwheat tea was also significantly different and the highest concentration of rutin was found in the whole embryo tea.

国家科技支撑计划项目（2012BAI27B07）；四川省科技支撑计划（2011SZ0233）；现代农业产业技术体系建设专项资金资助（CARS-08-D-3）；四川中医药管理局课题（2010-78）