蜡样芽孢杆菌D-11降解粉末壳聚糖制备水溶性低聚糖的研究

Preparation of Chito-oligosaccharides by Enzymatic Hydrolysis of Chitosan Powder

摘要:

为了省去制备胶状壳聚糖的工艺，能有效降解粉末壳聚糖利用到低聚糖的生产，利用自然环境中筛选得到的产壳聚糖酶菌株蜡样芽孢杆菌D-11，进行了基质条件优化实验。结果表明，以筛孔分别为20、40、60、80目的粉末壳聚糖（3±0.5 cm；脱乙酰度98.1%）为基质，培养液含有1%的吐温60时，降解粉末壳聚糖的能力比对照提高12%，当筛孔60目的粉末壳聚糖为碳源（浓度0.6%）时，降解粉末壳聚糖的能力提高62.5%。随着基质每毫克中酶活性的提高，对粉末壳聚糖的分解率也随之增加。同时利用薄层色谱法分析低聚糖的分布，有效证明了该D-11菌株具有降解粉末壳聚糖的能力，且发现壳聚糖酶对基质粉末状态的降解率有选择性。

Abstract:

In order to save the process of colloidal chitosan preparation in hydrolysis of chitosan powder to chito-oligosaccharide techniques. Chitosanse producing strains were isolated and identified as Bacillus cereus D-11. Twenty, forty, sixty and eighty meshes of powder chitosan (3±0.5 cm；D.D. 98.1%) were used as carbon sources to investigate the optimal culture conditions of this strain. The chitooligosaccharides products were analyzed by Thin Layer Chromatography. Compared to control, the enzymatic activity of Bacillus cereus D-11 was increased by 12% and 62.5% in a medium containing 1% Tween 60 or 60 mesh powder chitosan, respectively. The yield of chito-oligosaccharides positively correlated with the enzyme amount. In conclusion, the product of chitosan which was hydrolyzed by chitosanse was determinded as chitooligosaccharides by TLC analysis. The hydrolysis rate of chitoasan by chitosanse was determinded by size of chitosan.