金线莲菌根真菌的分离与鉴定

Isolation and Identification of Mycorrhizal Fungi from Anoectochilus

摘要:

从38株野生金线莲中共分离获得29株菌根真菌，其中丝核菌类（Rhizoctnia sp.）真菌7株，结合菌株形态、融合群测定及5.8S rDNA-ITS序列分析，鉴定结果如下：J1、F1、F7属于双核丝核菌AG-P融合群，F1、F7为AG-P融合群中的同一亚群，J1为不同的亚群；G1、G3、G5、G6为多核丝核菌，初步推断为兰科丝核菌类的念珠菌根菌（Moniliopsis）。

Abstract:

29 strains of endophytic fungi were isolated from A.roxburghii, and 7 strains belonged to Rhizoctnia sp. Basing on the culture modality of Rhizoctnia sp, tests of anastomosis group and analysis of 5.8S rDNA-ITS, J1, F1, F7 were found to belong to binucleate Rhizoctonia AG-P. F1and F7 were in the same sub-group, and J1 was in a single sub-group. G1, G3, G5 and G6 were pilot concluded belonging to Moniliopsis of multinucleate Rhizoctonia from orchid.