微波消解-电感耦合等离子体质谱法测定畜禽肉中的硼和铝

Determination of Aluminum and Boron in Livestock Products by Inductively Coupled Plasma Mass Spectrometry

摘要:

建立了畜禽肉中硼和铝元素的电感耦合等离子体质谱( ICP- MS)测定方法。样品用HNO3+H2O2+H2O经微波消解后，用ICP- MS进行分析。以Sc和Li作为内标物质，补偿了基体效应，优化仪器参数，考察了方法的检出限、线性范围、回收率和精密度等。结果表明：硼和铝在0~2.0 μg/mL线性范围内，线性关系良好，相关系数均大于0.999，铝和硼的方法检出限分别为70.5 ng/g和30.5 ng/g，相对标准偏差(n=6)分别为1.35%和2.01%；在5种不同样品基质（牛肉、猪肝、猪肚、牛百叶、鸡肉）的添加回收试验中，添加水平为0.3~3.2 μg/g时，两种元素的平均回收率为90.5%~107.2%。利用该方法分析鸡肉的国家标准物质，结果与标准值一致。所建立的方法简单、快速和准确，适用于检验分析畜禽肉中硼和铝元素。

Abstract:

A method was established to determination of Aluminum (Al) and Boron (B) in livestock products by inductively coupled plasma mass spectrometry (ICP-MS). After microwave digestion in HNO3 + H2O2 + H2O, the samples were determined directly by ICP-MS. The signal drift and matrix effect can be corrected by selecting Sc and Li as internal standard elements and the influence of mass spectral interference can be calibrated by optimizing the instrument conditions, the good linear relationships, recoveries and precisions were obtained. The calibration curves for Al and B were linear in the range of 0~2.0 μg/mL with correlation coefficients more than 0. 999. The quantization limit of Al and B of the method were 70.5 ng/g and 30.5 ng/g with RSD of 1.35% and 2.01% (n=6), respectively. The mean recoveries of Al and B from five spiked samples (Beef, Pig liver, Pig stomach, beef shutters, Chicken) at the spiking levels of 0.3~3.2 μg/g were ranged from 90.5% to 107.2%. Results showed that the Al and B values detected were in good agreement with the corresponding certified reference values chicken. The proposed method is fast, simple, sensitive and accurate, which can meet the demand for determining Al and B elements in livestock products.