ICP-MS法测定蜂花粉中的微量元素含量

Application Study on Determination of Trace Elements in Bee Pollens by Inductively Coupled Plasma Mass Spectrometry

摘要:

建立了蜂花粉中Na、Mg、Al、K、Ca、Cr、Mn、Fe、Cu、Zn、Co、Ni、As、Se、Sr、Mo、Cd、Sb、Ba和Pb共21种微量元素电感耦合等离子体质谱(ICP- MS)测定的分析方法。样品经微波消解后用ICP-MS进行分析。优化实验条件下，21种元素的线性范围为0~10 μg/mL，线性关系良好，相关系数均大于0.999，方法的检出限为0.029~148.3 ng/g，相对标准偏差(n=6)为0.6%~4.6%，添加水平为0.002~3000 μg/g时，蜂花粉样品中的21种元素平均加标回收率为91.7%~110%。应用于花粉的国家标准物质(GSB-17)，测定值与标准值的一致程度为91.2%~115.3%；方法具有简单、快速和准确的特点，是一种简便、快速分析蜂花粉中微量元素的方法。

Abstract:

A method was established to determination of Na, Mg, Al, K, Ca, Cr, Mn, Fe, Cu, Zn, Co, Ni, As, Se, Sr, Mo, Cd, Sb, Ba and Pb in bee pollens. After microwave digestion, the samples were determined directly by inductively coupled plasma mass spectrometry. Under the optimal conditions，the calibration curves for 21 trace elements were linear in the range of 0~10 μg/mL with correlation coefficients more than 0. 999. The quantization limit of the method were ranged from 0.029 ng/g to 148.3 ng/g with RSD of 0.6%~4.6% (n=6)．The mean recoveries of 21 trace elements from spiked samples at the spiking levels of 0.002~3000 μg/g were ranged from 91.7% to 110%. Results showed that the 21 trace elements values detected were in good agreement with the corresponding certified reference values inbee pollen (GSB-17), the degree of consistency were 91.2% to 115.3%. The proposed method is fast, simple, sensitive and accurate, which can meet the demand for determining 21 trace elements in bee pollens.