蛹虫草多糖的纯化及其分子量的测定

Isolation, Purification and Molecular Weight Determination of Polysaccharides from Cordyceps militaris

摘要:

以蛹虫草子实体为材料，对水提醇沉、硫酸锌盐去蛋白后获得蛹虫草粗多糖（CP）进行分离纯化。采用膜分离技术、DEAE-52柱层析及Sephadex G-100柱层析对CP进行分离纯化，并使用高效凝胶渗透色谱法（HPGPC）测定各组分多糖分子量，以表征不同路径所得多糖的分子量分布。结果表明：CP经膜过滤、DEAE-52柱层析及Sephadex G-100进一步柱层析得到多糖组分CP2-c2-s2，经HPGPC法鉴定，CP2-c2-s2为均一多糖组分，其平均分子量为20200 Da。

Abstract:

Crude polysaccharides (CP) were extracted from dried fruits of Cordyceps militaris (L.ex Fr) Link. through sequential steps of hot water extraction, ethanol precipitation and zinc sulfate deproteination. It was fractionated by membrane separation, purified by DEAE-cellulose-52 exchange chromatography and further purification by Sephadex G-100 gel filtration chromatography. Their molecular weights were determined using high performance gel-permeation chromatography (HPGPC) in order to characterize molecular weight distribution. The results showed that CP2-c2-s2 was a homogeneous polysaccharide with average molecular weight of 20200 Da, which could be obtained through aforementioned isolation and purification.