车前草多糖的脱色工艺研究

Decoloring Process of Polysaccharide Extracted from Plantago asiatica L

摘要:

以脱色率和多糖保留率为指标，采用活性炭和大孔吸附树脂两种方法对车前草多糖脱色。结果表明，活性炭脱色的最佳条件为：在60 ℃下，加入0.75%（m/V）的活性炭，脱色30 min，在此工艺条件下脱色率为76.22%，多糖保留率为65.31%。大孔吸附树脂脱色的最佳条件是：以蒸馏水为洗脱剂，样液pH值为8.0，洗脱流速为2 mL/min，洗脱液体积为3 BV（1 BV=20 mL），在此工艺条件下脱色率为79.78%，多糖保留率为89.76%。大孔吸附树脂脱色效果优于活性炭脱色效果。

Abstract:

The decoloring methods with active carbon and AB-8 macroporous adsorption resin were compared for polysaccharide extracted from Plantago asiatica L was decolored by analyzing the decoloration rate and polysaccharide retention rate. The results showed that the best conditions for decoloration with active carbon were: 0.75% active carbon, decoloring temperature 60 ℃ and treatment time 30min, under which the decolorization rate was 76.22% and the polysaccharide retention rate was 65.31%. The best conditions for AB-8 macroporous adsorption resin were as follows: the elution solvent 3 BV (1 BV=20 mL), flow rate 2 mL/min, and pH of polysaccharide solution 8.0, under which the decolorization rate was 79.78% and the polysaccharide retention rate was 89.76%. The decoloration with macroporous adsorption resin was better than that with active carbon.