汉麻籽壳多酚的提取及抗氧化活性评价

Extraction and Anti-oxidant Activity Evaluation of Polyphenol from Hempseed Hull

摘要:

汉麻籽壳是汉麻籽加工的副产物，其中含有丰富的抗氧化物质。本文以汉麻籽抗氧化多酚得率为指标，筛选出汉麻籽壳抗氧化多酚提取的适宜条件，并对提取物的抗氧化性能进行评价。结果表明，汉麻籽壳中抗氧化成分最适宜提取的条件为液料比（10:1）、提取时间（30 min）、提取次数（3次）、提取液浓度（60%乙醇溶液）；市售大孔树脂中HPD-600对汉麻籽壳抗氧化成分的吸附能力最强；汉麻籽壳中抗氧化成分最适宜的洗脱条件为洗脱流速（1 mL/min）的梯度洗脱，40%乙醇洗脱溶液为目标产物；经过大孔树脂分离提取的汉抗氧化多酚对于质粒DNA、人血低密度脂蛋白（LDL）和牛血清蛋白（BSA）的氧化损伤都有显著的保护作用。

Abstract:

Hempseed hull, a by-product in hempseed oil processing, is rich in antioxidants. In this study, the conditions of polyphenol extraction from hempseed hull by ultrasonic were optimized on base of yield, and in vitro antioxidant properties of the extract were evaluated. Solvent extraction and macroporous resin absorption were employed in polyphenol separation. The results showed that the optimal extraction conditions were as follows: solid-liquid ratio 1:10, extraction time 30 min, extraction times 3 times and ethonal concentration 60%. HPD-600 was determined as the optimal macroporous resins for hempseed hull antioxidant polyphenol extraction and elunet by 40% ethanol was the target extract with the highest antioxidant activity and yield. The extract presented significant protective capacities of human low density lipoprotein (LDL), protein, and DNA against oxidation evaluated by electrophoresis. The hempseed hull extract was valuable in antioxidant properties and can be utilized as a natural antioxidant dietary supplement.