不同种类低值鱼酶解效果的比较及优化

Comparison of Enzymatic Hydrolysis Effect on Different Kinds of Trash Fish and the Optimization of Hydrolysis Conditions

摘要:

本文对比了不同海洋低值鱼的蛋白质含量和酶解效果，并对青鳞鱼和斑鰶的酶解方案进行优化。结果表明：蓝园鲹和青鳞鱼的蛋白质含量较高，为17%以上；斑鰶、黄鲫、刺鲳、鲻鱼、鳓鱼的蛋白质含量为15~16%。但蓝园鲹、黄鲫、刺鲳、鲻鱼的酶解效果较好，青鳞鱼、斑鰶和鳓鱼的酶解效果较差。青鳞鱼的最佳酶解方案为：木瓜蛋白酶0.05%，风味蛋白酶0.1%，水解蛋白酶0.05%，胰蛋白酶0.15%，可提高蛋白质利用率为86.07%，氨基酸转化率为49.24%，酶解清液出品量为11.30 mL。斑鰶的最佳酶解方案为：木瓜蛋白酶0.15%，风味蛋白酶0.1%，水解蛋白酶0.05%，胰蛋白酶0.15%，可提高蛋白质利用率为83.22%，氨基酸转化率为49.14%，酶解清液出品量为10.91 mL。

Abstract:

The content of proteins and the enzymatic hydrolysis effects on different kinds of trash fishes were compared. And the enzymatic hydrolysis effects of Hanergula zunasi and Clupanodon punctatus were be modified. The results showed that the protein contents of Decapterus maruadsi and Hanergula zunasi are more than 17%, and for Clupanodon punctatus, Setighnna taty，Hanergula zunasi，Mugil cephalus and Llisha elongata，the protein contents were ranged in 15-16%. The enzymatic hydrolysis effects of Decapterus maruadsi, Setighnna taty, Hanergula zunasi and Mugil cephalus were better than those of Hanergula zunasi, Clupanodon punctatus and Llisha elongate. The best enzymes for Hanergula zunasi hydrolysis are the mixture of 0.05% papain, 0.1% Flavourzyme, 0.05% Alcalase and 0.15% trypsin, with which 86.07% of protein recovery, 49.24% of degree ofhydrolysis and 11.30 ml of the production quantity were achieved. The best enzymes for Clupanodon punctatus were the mixute of 0.15% papain, 0.1% Flavourzyme, 0.05% Alcalase and 0.15% trypsin, with which 83.22% of protein recovery, 49.14% of degree ofhydrolysis and 10.91 ml of the production quantity were achieved.