气相色谱-三重串联四极杆质谱法测定粮谷中169种农药的残留量

Determination of 169 Pesticide Residues in Grains by Gas Chromatography Tandem Mass Spectrometry

摘要:

建立了粮谷中169种农药多残留的气相色谱-三重串联四极杆质谱(GC-MS/MS)的检测方法。样品经改进的QuEChERS净化处理，气相色谱分离，串联四级杆质谱多反应监测方式检测。结果表明，169种农药在粮谷中的检出限为0.3~13.0 μg/kg，定量限为1.0~40.0 μg/kg，线性范围在0.005 mg/L~2.0 mg/L之间，线性相关系数大于0.99，平均回收率在65.3%~143.6%之间，97%的农药相对标准偏差小于20%，该方法具有前处理简单、净化效果好、灵敏度高的特点，适用于粮谷中多组分农药残留的快速确认和定量检测。

Abstract:

A method was developed for the simultaneous determination of 169 pesticides in grains by gas chromatography with tandem mass spectrometry (GC-MS/MS). The homogenized sample was extracted with acetonitrile, and then purified by QuEChERS, The targeted compounds were detected by GC-MS/MS system under the mode of multiple reaction monitoring (MRM). The results indicated that the limits of detection (LODs) of 169 pesticides were ranged from 0.3 to 13.0 μg/kg. The LOQs of the tested pesticides were between 1.0 and 40.0 μg/kg. In the linear range between 0.005 and 2.0 mg/L of each pesticide, the linear correlation coefficients were greater than 0.99. The average recoveries ranged from 65.3% to 143.6% and the RSDs of 97% pesticides were less than 20%. This method was suitable for the identification and quantification of multi-component pesticides in grains due to its simplicity, good purification and high sensitivity.