高效液相色谱法检测蔬菜中的吡虫啉残留

Determination of Imidaeloprid Pesticide Residue in Fresh Vegetables by HPLC

摘要:

本文建立了一种果蔬中吡虫啉农药残留的高效液相色谱检测方法。样品经乙腈提取、盐析、LC-NH2小柱净化，采用高效液相色谱仪-紫外检测器进行测定，色谱柱：Waters Symmetry Shield? C18（4.6×250 mm，5 μm）；柱温：30.0 ℃；检测波长：275 nm；流动相：甲醇:水:乙腈=55:25:20（体积比）；流速：0.8 mL/min；进样量：10.0 μL。吡虫啉在0.1 mg/L至1.0 mg/L的添加范围内线性良好，线性关系系数r为0.9925；添加水平为0.05~0.20 mg/kg时，西红柿样品的平均回收率为95.4%~106.8%，相对标准偏差为2.66%~7.99%。结果表明该方法具有快速、灵敏、准确等优点，适合蔬菜中吡虫啉农药残留的测定。

Abstract:

A novel high performance liquid chromatography (HPLC) method was developed for the analysis of imidaeloprid pesticide residue in fresh vegetables. Imidaeloprid was extracted from the samples by acetonitrile, purified with LC-NH2 column and determined by high performance liquid chromatography with UV detection. Symmetry Shield? C18 column (4.6×250 mm, 5 μm) using the mixture of methanol, water and acetonitrile (55:25:20) as the mobile phase. The column temperature, flow rate，detection wavelength and sample volume were 30.0 ℃, 0.8 mL/min, 275 nm and 10.0 μL, respectively. Within the linear range of 0.1~1.0 mg/L, the correlation coefficient, the recoveries of imidaeloprid in tomato and the relative standard deviations (RSD) were 0.9925, 95.4%~106.8 % and 2.66%~7.99%, respectively. The method was simple, effective and accurate. It can be applied to determinate imidaeloprid pesticide residue in fresh vegetables.