通过对伍仁、蛋黄莲蓉、枣泥、水果、火腿、冰皮和吞拿鱼七种口味，共70批次月饼进行甲醇-水（1:1，V/V）和三氯甲烷二次抽提两种处理方法对比，结果显示两种方法检测仅伍仁和吞拿鱼月饼差异显著，其余不显著。甲醇水提取方法检测结果重复性良好，加标回收率为84.0%~114.7%，提高了月饼黄曲霉毒素B1的检测效率。

A study was conducted to optimize extraction method of Aflatoxin B1 from eight kinds of moon cake (mixed nuts, lotus seed with egg yolk, jujube paste, fruit, ham, snowy, and tuna) through ELISA. The detection results by two methods showed no significant difference except the detection in Mixed Nuts and Tuna. Better extraction efficacy was found using 50% methanol/water solution. The average recovery rate was 84.0%~114.7%. The method could improve the detection efficiency of aflatoxin B1 of moon cake.