胶体金免疫层析试纸条快速检测水和玉米中阿特拉津的残留

Rapid Detection of Atrazine Residue in Water and Corn by Colloidal Gold-based Immunochromatographic Strip

摘要:

本文以胶体金作为抗体标记物，建立了一种检测阿特拉津残留的免疫层析试纸条快速检测方法。该方法的检出限为2 ng/mL，10 min内可以得到检测结果。本文以自来水和玉米作为样品进行了添加回收实验，水样不需要任何前处理直接检测，水样的检出限为2 ng/mL；玉米样品经简单前处理后检测，玉米样品的检出限为40 ng/g，且该方法的检测结果与间接竞争ELISA方法的结果一致。该方法具有成本低、操作简单、灵敏度高、检测快速，结果易于判定等优点，适用于大量样品的现场筛选。

Abstract:

An immunochromatographic strip assay using colloidal gold-labeled antibody was developed. The visual limit of detection (LOD) of the assay for atrazine in PBS was 2 ng/mL. And the results could be obtained within 10 min. Spiked tap water sample was analyzed directly by the test strip without any pretreatment and the LOD was 2 ng/mL. Meanwhile, spiked corm sample was analyzed after simple pretreatment and the LOD was 40 ng/g. The results obtained by the immunochromatographic strip assay correlated well with those determine by indirect competitive ELISA. Because of its low cost, simple operation, high specificity, fast detection and easy-to-judgment results, the strip could serve as an on-site screening of a large number of samples.