CHU-R菌株产虾青素的发酵工艺研究

Fermentation of CHU-R for Astaxanthin Production

摘要:

本文通过对获得的虾青素产生菌CHU-R进行最佳培养基、培养温度、接种量、初始pH等发酵工艺参数的优化研究，得到CHU-R菌产虾青素的最佳培养基为：6%蔗糖、1%糖蜜、0.5%硫酸铵、0.3% Na2HPO4、0.05% KI、0.05% MgSO4?7H2O；50 L罐最适发酵工艺条件为：温度28 ℃，通风量1:0.1~0.15（V/V/min）、初始转速120 r/min、罐压0.05~0.08 MPa、装液量30 L、初始pH 6.8~7.0、接种量6%，发酵过程维持溶氧量在40~60%之间。由此得到的虾青素含量近达23 mg/g干菌体重。

Abstract:

The fermentation process parameters of CHU-R, such as medium, incubation temperature, inoculation, etc. were discussed in this paper. It was found that the best medium formulas was: 6% sucrose, 1% molasses, 0.5% ammonium sulfate, 0.3% Na2HPO4，0.05% KI，and 0.05% MgSO4?7H2O. The optimum fermentation conditions in 50 L fermenter were: temperature 28℃, venti1ation 0.1~0.15 (V/V/min), the initial speed of 120rpm, tank pressure 0.05~0.08 MPa, liquid volume 30 L, the initial pH 6.8~7.0 and inoculum size 6%, the fermentation process to maintain dissolved oxygen at 40-60%. The results showed that the CHU-R yield pigment nearly reached 23 mg/g of its dry weigh fermented in 50 L fermenter.