化妆品中糖皮质类激素的快速测定

Rapid Determination of Glucocorticosteroids in Cosmetics

摘要:

建立了一种简单、快速测定化妆品中三十种糖皮质类激素的分析方法。采用饱和氯化钠溶液-乙腈-正己烷三相溶剂直接提取并净化样品，亚铁氰化钾-醋酸锌溶液沉淀乳化基质后，用超高效液相色谱进行测定。另外，本文对不同的提取溶剂和提取方法的提取效果等做了系统的比较，优化了实验条件，方法的回收率为89.8%~105.2%，相对标准偏差为5.0%~10.3%，方法定量限为2.0 mg/kg。

Abstract:

A simple and rapid method was developed for determination of 30 specified glucocorticosteroids. Sample was extracted and purified by three phases solvent system comprised of saturated aqueous sodium chloride solution, acetonitrile and hexane. After emulsified matrix was precipitated by potassium ferrocyanide and zinc acetate solution, 30 specified glucocorticosteroids were determined by Ultra Performance Liquid Chromatography (UPLC). In addition, extraction conditions were further optimized by systematically reseaching on effects of different extracting solvents and methods. With this method, the average recoveries for glucocorticosteroids ranged from 89.8 % to 105.2 %; The relative standard deviations were between 5.0 % and 10.3 %; And the method quntification limit was 2.0 mg/kg.