纤维素酶酶解玉米秸秆新型蒸煮浆的工艺研究

Research of Enzymatic Hydrolysis of Corn Stalk Pulp with New Cooking Method

摘要:

本文以玉米秸秆活性氧固体碱蒸煮浆（木素脱除率82.1%）为原料，用国产纤维素酶（酶活为13.94 FPU/100 mL）进行酶解试验，探索酶解最佳工艺条件。结果显示：底物固液比为0.47%（m/V），酶解pH值为4.70，酶解温度为50 ℃，酶解时间为72 h时酶解液还原糖含量最大，即糖得率最大，达59.41%。本研究也进行玉米秸秆经不同方法预处理后的酶解实验，结果显示其纤维素酶解效果顺序为：活性氧固体碱蒸煮浆＞硫酸盐浆＞机械粉碎＞未预处理玉米秸秆。

Abstract:

The objective of this study was to explore technique of enzymatic hydrolysis of corn stalk pulp (removal rate of lignin of 82.1%) from active oxygen cooking with a solid alkali with Chinese cellulase (enzyme activity: 13.94 FPU/100ml). The results showed that the optimum process conditions were the following: enzyme-substrate ratio of 0.47% (m/V), pH value of 4.70， temperature of 50℃ and enzymatic reaction time of 72 h. The highest yield of sugar was 59.41% under the optimum process conditions. The paper also presented the different enzymolysis of corn stalk pretreated by different methods, and the order of the effect of enzymolysis was as follows: corn stalk pulp from active oxygen cooking with a solid base > corn stalk pulp from alkaline cooking >> corn stalk pretreated by mechanical crushing > raw corn stalk.