菌株BS-3的鉴定及其真菌抑制活性的初步研究

Identification of the Strain BS-3 and Preliminary Study on the Characterization of the Antifungal Substance

摘要:

从土壤中分离得到一株对多种植物病原真菌具有抑制作用的菌株，将其命名为BS-3。该菌株对水稻纹枯病、稻瘟病、苦瓜枯萎病等病原真菌均有较强抑制作用。通过对菌株BS-3的16S rDNA的序列测定和同源性比对，发现它与Bacillus licheniformis、Bacillus subtilis、Bacillus amyloliquefaciens等的16S rDNA有高达100%的同源性，结合形态和生理生化鉴定指标，将其鉴定为地衣芽孢杆菌Bacillus licheniformis。选用受其抑制作用较明显的水稻纹枯病病原真菌瓜亡革菌（Thanatephorus cucumeris）作为指示菌，牛津杯法检测BS-3菌株发酵产物的抑菌活性。结果表明BS-3菌株的发酵上清液滤液、硫酸铵处理离心沉淀对水稻纹枯病菌有较强抑菌活性。此外，该抗真菌物质具有较强热稳定性。

Abstract:

A strain of inhibition to many kinds of plant pathogenic fungi, named BS-3, was isolated from soils. The strain had quite strong fungistatic activities on plant pathogenic fungi such as rice sheath blight, rice blast. It had 100% homologous to Bacillus licheniformis, Bacillus subtilis and Bacillus amyloliquefaciens by 16S rDNA sequences BLAST analysis plus the results of morphological and biochemical parameters. It was confirmed as a strain of Bacillus licheniformis. Using rice sheath blight fungus Thanatephorus cucumeris as the indicator bacteria, inhibition zone method was chosen to detect antifungal activity of fermentation product belong to the strain BS-3. The results showed that the fermentation liquor after the treatment of centrifuging and 50% saturated ammonium sulfate had quite strong fungistatic activities. In addition, the antifungal substances had strong thermostability.